Effects of Gamma Ray Irradiation to Induce Genetic Variability of Teak Planlets (Tectona grandis Linn. F.)

نویسندگان

چکیده

Teak planlets (Tectona grandis) of Salomon clones were irradiated by gamma rays to induce genetic variability for growth improvement. The objectives this research analyze the radiosensitivity T. grandis ray irradiation and variations using Random Amplified Polymorphic Deoxyribonucleic Acid (RAPD). For those purposes, teak at 5 different dosages, 0 Gy, 10 20 30 40 Gy. Lethal doses50 (LD50) reduced (RD50) obtained Curve-fit Analysis. Furthermore, subcultured on medium MS + 0.1 kinetin (M1V0) surviving M1V0 plantlets multiplied kinetin, so it is M1V1 generation. Genetic variation mutant was molecularly analyzed RAPD methods calculated Analysis Molecular Variance (AMOVA). Results showed that lethal values 24.5 Gy 7.85 respectively. It includes genomes small size a number genomes. individuals in among within treatment 16% 84% according AMOVA. means dominant effect material caused generations. This dominance could be response combination tissue culture factors had potency used as materials select desired next stage.

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ژورنال

عنوان ژورنال: Journal of Sylva Indonesiana

سال: 2022

ISSN: ['2622-5093', '2622-5158']

DOI: https://doi.org/10.32734/jsi.v5i01.6166